{"id":3821,"date":"2018-12-18T13:41:03","date_gmt":"2018-12-18T12:41:03","guid":{"rendered":"http:\/\/www.igmm.cnrs.fr\/?p=3821"},"modified":"2018-12-18T16:20:32","modified_gmt":"2018-12-18T15:20:32","slug":"le-facteur-pionner-zelda-controle-le-timing-dactivation-des-genes-via-des-interactions-transitoires-dans-des-microenvironnements-nucleaires","status":"publish","type":"post","link":"https:\/\/www.igmm.cnrs.fr\/en\/le-facteur-pionner-zelda-controle-le-timing-dactivation-des-genes-via-des-interactions-transitoires-dans-des-microenvironnements-nucleaires\/","title":{"rendered":"Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs"},"content":{"rendered":"<div style=\"width: 512px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-3821-1\" width=\"512\" height=\"512\" autoplay preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/mp4-m92.mp4?_=1\" \/><a href=\"http:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/mp4-m92.mp4\">http:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/mp4-m92.mp4<\/a><\/video><\/div>\n<p>&nbsp;<\/p>\n<p>Pioneer transcription factors can engage nucleosomal DNA, which leads to local chromatin remodeling and to the establishment of transcriptional competence. However, the impact of enhancer priming by pioneer factors on the temporal control of gene expression and on mitotic memory remains unclear. Here we employ quantitative live imaging methods and mathematical modeling to test the effect of the pioneer factor Zelda on transcriptional dynamics and memory in Drosophila embryos. We demonstrate that increasing the number of Zelda binding sites accelerates the kinetics of nuclei transcriptional activation regardless of their transcriptional past. Despite its known pioneering activities, we show that Zelda does not remain detectably associated with mitotic chromosomes and is neither necessary nor sufficient to foster memory. We further reveal that Zelda forms sub-nuclear dynamic hubs where Zelda binding events are transient. We propose that Zelda facilitates transcriptional activation by accumulating in microenvironments where it could accelerate the duration of multiple pre-initiation steps.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-3798 aligncenter\" src=\"http:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/Imagesiteweb-450x255.png\" alt=\"\" width=\"450\" height=\"255\" srcset=\"https:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/Imagesiteweb-450x255.png 450w, https:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/Imagesiteweb-768x436.png 768w, https:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/Imagesiteweb-1024x581.png 1024w, https:\/\/www.igmm.cnrs.fr\/wp-content\/uploads\/2018\/12\/Imagesiteweb.png 1080w\" sizes=\"auto, (max-width: 450px) 100vw, 450px\" \/><\/p>\n<p><strong>Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs.<\/strong><br \/>\nDufourt J, Trullo A, Hunter J, Fernandez C, Lazaro J, Dejean M, Morales L, Nait-Amer S, Schulz KN, Harrison MM, Favard C, Radulescu O, Lagha M.<br \/>\nNat Commun. 2018 Dec 5;9(1):5194. doi: 10.1038\/s41467-018-07613-z.<br \/>\n<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Temporal+control+of+gene+expression+by+the+pioneer+factor+Zelda+through+transient+interactions+in+hubs\">https:\/\/doi.org\/10.1038\/s41467-018-07613-z<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pioneer transcription factors can engage nucleosomal DNA, which leads to local chromatin remodeling and to the establishment of transcriptional competence<\/p>\n","protected":false},"author":4,"featured_media":3798,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,16],"tags":[],"class_list":["post-3821","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-news","category-science-en"],"_links":{"self":[{"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/posts\/3821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/comments?post=3821"}],"version-history":[{"count":5,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/posts\/3821\/revisions"}],"predecessor-version":[{"id":3837,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/posts\/3821\/revisions\/3837"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/media\/3798"}],"wp:attachment":[{"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/media?parent=3821"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/categories?post=3821"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.igmm.cnrs.fr\/en\/wp-json\/wp\/v2\/tags?post=3821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}