Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere synthesis, telomerase has not yet been detected at telomeres. The RNA component of human telomerase (hTR) resides in the nucleoplasmic Cajal bodies (CBs) of interphase cancer cells. Here, in situ hybridization demonstrates that in human HeLa and Hep2 S phase cells, besides accumulating in CBs, hTR specifically concentrates at a few telomeres that also accumulate the TRF1 and TRF2 telomere marker proteins. Surprisingly, telomeres accumulating hTR exhibit a great accessibility for in situ oligonucleotide hybridization without chromatin denaturation, suggesting that they represent a structurally distinct, minor subset of HeLa telomeres. Moreover, we demonstrate that more than 25% of telomeres accumulating hTR colocalize with CBs. Time-lapse fluorescence microscopy demonstrates that CBs moving in the nucleoplasm of S phase cells transiently associate for 10-40 min with telomeres. Our data raise the intriguing possibility that CBs may deliver hTR to telomeres and/or may function in other aspects of telomere maintenance.
Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres
Jady, B. E.; Richard, P.; Bertrand, E.; Kiss, T.
Molecular Biology of the Cell
2006-02 / vol 17 / pages 944-954
IGMM team(s) involved in this publication
Biogenèse des ARNs
mammalian-cells; protein; body-specific localization; box h/aca scarnas; human cancer; human-chromosomes; length regulation; nuclear-matrix; promyelocytic leukemia bodies; repetitive DNA-sequences