We have compared the sequences of the nine human immunoglobulin V lambda gene subgroups in order to define specific sequences for the IGLV9S1 gene. The oligonucleotides corresponding to these regions, in both forward and reverse positions, were used in polymerase chain reactions from human genomic DNA. A unique fragment of 177 base pairs was amplified from positions 136-312 of the IGLV9S1 gene and cloned in pUC18. When used as a probe in Southern hybridization with human genomic DNA, a unique band was detected, indicating that IGLV9S1 is a single-copy gene. We have defined this fragment as a sequence-tagged site designated IGLV9S1 [/22q11] for the IGL locus.
Definition of a sequence-tagged site for the human immunoglobulin IGLV9S1 gene located at chromosome 22q11
Passos, G. A.; Frippiat, J. P.; Lefranc, M. P.
Exp Clin Immunogenet
1994 / vol 11 / pages 222-6
Humans; Amino Acid Sequence; Molecular Sequence Data; Blotting, Southern; Polymerase Chain Reaction; Base Sequence; Restriction Mapping; *Chromosomes, Human, Pair 22; *Sequence Tagged Sites; Immunoglobulin lambda-Chains/*genetics