In the present study, we have investigated the anatomic distribution in blood and gut mucosal tissues of memory poxvirus-specific CD4 and CD8 T cells in subjects vaccinated with smallpox and compared it with vector (NYVAC)-specific and HIV insert-specific T-cell responses induced by an experimental DNA-C/ NYVAC-C vaccine regimen. Smallpox-specific CD4 T-cell responses were present in the blood of 52% of the subjects studied, while smallpox-specific CD8 T cells were rarely detected (12%). With one exception, smallpox-specific T cells were not measurable in gut tissues. Interestingly, NYVAC vector-specific and HIV-specific CD4 and CD8 T-cell responses were detected in almost 100% of the subjects immunized with DNA-C/NYVAC-C in blood and gut tissues. The large majority (83%) of NYVAC-specific CD4 T cells expressed alpha4beta7 integrins and the HIV coreceptor CCR5. These results demonstrate that the experimental DNA-C/NYVAC-C HIV vaccine regimen induces the homing of potentially protective HIV-specific CD4 and CD8 T cells in the gut, the port of entry of HIV and one of the major sites for HIV spreading and the depletion of CD4 T cells.
DNA/NYVAC vaccine regimen induces HIV-specific CD4 and CD8 T-cell responses in intestinal mucosa
Perreau, M.; Welles, H. C.; Harari, A.; Hall, O.; Martin, R.; Maillard, M.; Dorta, G.; Bart, P. A.; Kremer, E. J.; Tartaglia, J.; Wagner, R.; Esteban, M.; Levy, Y.; Pantaleo, G.
2011-10 / vol 85 / pages 9854-62
JVI.00788-11 [pii] 10.1128/JVI.00788-11
1098-5514 (Electronic) 0022-538X (Linking)
IGMM team(s) involved in this publication
Eric J Kremer
Adénovirus : Récepteurs, Trafic Intracellulaire et Vectorologie
Humans; Adult; Middle Aged; AIDS Vaccines/administration & dosage/*immunology; Blood/immunology; CD4-Positive T-Lymphocytes/*immunology; CD8-Positive T-Lymphocytes/*immunology; Intestinal Mucosa/*immunology; Smallpox Vaccine/administration & dosage/*immunology; Vaccination/methods; Vaccines, DNA/administration & dosage/*immunology; Viral Vaccines/administration & dosage/*immunology