OBJECTIVES: Interleukin (IL)-10 has anti-atherogenic properties. However, the molecular mechanisms involved in IL-10 protection against atherosclerosis in vivo remain poorly understood. In this study, we examined the effect of IL-10 cDNA in vivo electrotransfer on diet-induced, endothelial activation. METHODS: C57BL/6J mice were fed an atherogenic diet for 10 days. Expression of VCAM-1 and ICAM-1 was examined in the aortic sinus, a region predisposed to atherogenesis in mice, using immunohistochemistry. NF-kappaB activation was examined using a monoclonal antibody that selectively reacts with the activated form of the p65 subunit. RESULTS: We detected a low basal expression of activated NF-kappaB, VCAM-1 and ICAM-1 in the endothelium of the aortic sinus. Endothelial expression of activated NF-kappaB, VCAM-1 and ICAM-1 was markedly increased after 10 days on the atherogenic diet (p < 0.001). In vivo electrotransfer of a murine IL-10-encoding plasmid completely prevented diet-induced endothelial upregulation of activated NF-kappaB, VCAM-1 and ICAM-1 (p < 0.01). CONCLUSION: In vivo electrotransfer of IL-10 cDNA prevents diet-induced endothelial activation. These results suggest that the protective effects of IL-10 may already occur in the very early stages of atherogenesis.
In vivo electrotransfer of interleukin-10 cDNA prevents endothelial upregulation of activated NF-kappaB and adhesion molecules following an atherogenic diet
Potteaux, S.; Deleuze, V.; Merval, R.; Bureau, M. F.; Esposito, B.; Scherman, D.; Tedgui, A.; Mallat, Z.
Eur Cytokine Netw
2006-03 / vol 17 / pages 13-8
Female; Animals; Mice; Mice, Inbred C57BL; Enzyme Activation; Up-Regulation; Gene Transfer Techniques; Electroporation; DNA, Complementary; *Diet, Atherogenic; Endothelium/*metabolism; Intercellular Adhesion Molecule-1/*biosynthesis; Interleukin-10/genetics/*metabolism; NF-kappa B/*biosynthesis; Sinus of Valsalva/*metabolism; Vascular Cell Adhesion Molecule-1/*biosynthesis