The maps of the human immunoglobulin heavy-chain and kappa light-chain loci have recently been completed. We have now completed a map of the human lambda locus (IGL) located on chromosome 22q11.2. We mapped 52 V lambda genes from 10 V lambda families and 7 J lambda and C lambda genes on a 1140 kb contig constructed from eight YACs and 129 cosmid clones. The V lambda genes are arranged within 800 kb. Genes of the different V lambda families are organized in three clusters, V lambda II and III families (cluster A); V lambda I, V, VII and IX families (cluster B); V lambda IV, VI, VIII and X families (cluster C), in contrast to the dispersed organization of the different VH and V kappa families within the human VH and V kappa loci. We note that the most frequently used V lambda families (V lambda II and III) are proximal to the J lambda and C lambda genes. The VpreB gene, encoding part of the surrogate light chain, the GGT2 gene and the BCRL4 pseudogene were also mapped within the lambda locus.
Organization of the human immunoglobulin lambda light-chain locus on chromosome 22q11.2
Frippiat, J. P.; Williams, S. C.; Tomlinson, I. M.; Cook, G. P.; Cherif, D.; Le Paslier, D.; Collins, J. E.; Dunham, I.; Winter, G.; Lefranc, M. P.
Hum Mol Genet
1995-06 / vol 4 / pages 983-91
Humans; Molecular Sequence Data; Base Sequence; DNA Primers; Haplotypes; Chromosome Mapping; *Chromosomes, Human, Pair 22; Immunoglobulin lambda-Chains/*genetics; Electrophoresis, Gel, Pulsed-Field