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Hematopoiese Moléculaire

Eric Soler

Projets de recherche

The major goal of the laboratory is to understand how dynamic 3D genome organization provides a framework for efficient control of gene expression in time and space.

We use hematopoiesis, and specifically eythropoiesis, as a biological system to study how spatial genome conformation evolves during cellular differentiation and development. We aim at dissecting the molecular events controlling chromosome topology and fine tuning of gene expression in normal hematopoiesis and human pathological disorders such as genetic erythroid diseases and leukemia.

We use combination of functional genomics and proteomic tools to dissect the role of transcription factor complexes and chromatin regulatory factors in the establishment of chromatin looping, allowing long-range connections between genes and their distal enhancers. In particular we develop:
  • Chromosome Conformation Capture technologies, such as 4C-Seq and Hi-C,
  • Chromatin Immunoprecipitation-Sequencing, such as ChIP-Seq and related techniques,
  • Proteomics analysis of chromatin associated complexes, using IP-MS and Bio-ID,
  • Crispr/Cas approaches for genome editing and generation of knock-in alleles.

This set of complementary approaches is used on mouse and human hematopoietic cells lines, ex vivo differentiated primary human cells and novel mouse models to shed light on the dynamic regulatory network controlling hematopoietic differentiation and erythropoiesis.

Membres

Team leader

Eric SOLER

Chercheur DR2

+33 (0)4 34 35 96 55

117

Charlotte ANDRIEU-SOLER

CRCN

+33 (0)4 34 35 96 57

117

Sanjay CHAHAR

Post-Doc

+33 (0)4 34 35 9657

Virginie DELEUZE

CRCN

(+33) 04 34 35 96 57

117

Cindy GENOUD

IE-Recherche

+33 (0)4 34 35 96 55

Henry NEVEU

Stagiaire

+33 (0)4 34 35 96 57

117

Sylvain ROQUE

IE-Recherche

+33 (0)4 34 35 96 55

MOHAMMAD SALMA

IE-Recherche

+33 (0)4 34 35 96 57

117

Sélection de publications

Autres informations

Financement

Labex EpiGenMed Installation grant (2017-2019)

Labex GR-Ex (2016-2017)

Atip-Avenir fellowship (2014-2018)

FSER foundation (2015-2016)

ANR Blanc (2017-2019)

INCa PLBIO (2017-2019)

Interactions
At IGMM

Naomi Taylor

Jean-Christophe Andrau

 

National

Olivier Hermine (Imagine Institute, Necker Hospital, Paris)

Salvatore Spicuglia (TAGC, Marseille)

Thomas Mercher (Gustave Roussy, Villejuif)

Françoise Pflumio (Inserm/CEA, Fontenay-aux-Roses)

Paul-Henri Roméo (Inserm/CEA, Fontenay-aux-Roses)

Michaela Fontenay (Cochin Institute, Paris)

Toutes les équipe de recherche

Mots-clés
Model organism studied
Mouse (cells, embryo, adult organism), Primary Human Stem/progenitor cells, model cell lines
Biological process
Chromatin organization during normal and pathological erythroid differentiation (thalassemia, leukemia)
Biological techniques
Chromosome Conformation Capture technologies (4C-Seq, Hi-C), High-throughput chromatin and transcriptome profiling assays (ChIP-Seq, DNAse-Seq, RNA-Seq), Exome-Seq, Xenograft experiments (Human leukemia), Bioinformatics