A novel subgenomic murine leukemia virus RNA transcript results from alternative splicing

Dejardin, J.; Bompard-Marechal, G.; Audit, M.; Hope, T. J.; Sitbon, M.; Mougel, M.

J Virol

2000-04 / vol 74 / pages 3709-14


Here we show the existence of a novel subgenomic 4.4-kb RNA in cells infected with the prototypic replication-competent Friend or Moloney murine leukemia viruses (MuLV). This RNA derives by splicing from an alternative donor site (SD’) within the capsid-coding region to the canonical envelope splice acceptor site. The position and the sequence of SD’ was highly conserved among mammalian type C and D oncoviruses. Point mutations used to inactivate SD’ without changing the capsid-coding ability affected viral RNA splicing and reduced viral replication in infected cells.

Lire sur PubMed


Animals; Cells, Cultured; Mice; Virus Replication; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic; Mutation; *Alternative Splicing; Capsid/genetics; Friend murine leukemia virus/*genetics/metabolism/physiology; Moloney murine leukemia virus/*genetics/metabolism/physiology; RNA, Viral/*genetics/metabolism

Toutes les publications