Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex

Marin, M.; Brockly, F.; Noel, D.; Etienne-Julan, M.; Biard-Piechaczyk, M.; Hua, T. D.; Gu, Z. J.; Piechaczyk, M.


1995-10 / vol 14 / pages 443-51


B9.12.1 is a monoclonal antibody specific for a monomorphic determinant of human MHC class I molecules. It is currently used for cell typing and is useful for targeting infection of human cells by murine ecotropic retroviruses. We have cloned and expressed it in the form of a single-chain variable fragment (ScFv) that recognizes the same epitope as the parental antibody. Through genetic engineering, this ScFv may be used for developing new cell-typing probes and new retroviral targeting approaches.

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Humans; Amino Acid Sequence; Molecular Sequence Data; Base Sequence; Cloning, Molecular; *Genes, Immunoglobulin; *Antibody Specificity; Binding Sites, Antibody; Epitopes/chemistry/*immunology; Gene Expression/immunology; Genes, MHC Class I/immunology; Histocompatibility Antigens Class I/*genetics/*immunology; Immunoglobulin Variable Region/biosynthesis/*genetics/isolation &; purification

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