Pharmacokinetics of oligonucleotides in cell culture

Lebleu, B.; Robbins, I.; Bastide, L.; Vives, E.; Gee, J. E.

Oligonucleotides as Therapeutic Agents

1997 / vol 209 / pages 47-54


Synthetic oligonucleotides offer interesting perspectives for the regulation of gene expression in normal and pathological situations. Poor uptake in many cell types, inadequate intracellular compartmentalization, often fragmentary knowledge of intracellular behaviour and mechanism of action, and lack of specificity remain major challenges. These limitations strongly urge the design of new oligonucleotide analogues and more efficient antisense strategies. Present achievements and perspectives for further developments will be discussed with emphasis on cell delivery and intracellular fate.



gene-transfer; proteins; membrane; antisense oligonucleotides; delivery; oligodeoxynucleotides; DNA; inhibition; helix; peptide

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