The exon-junction-complex-component metastatic lymph node 51 functions in stress-granule assembly

Baguet, A.; Degot, S.; Cougot, N.; Bertrand, E.; Chenard, M. P.; Wendling, C.; Kessler, P.; Le Hir, H.; Rio, M. C.; Tomasetto, C.

Journal of Cell Science

2007-08-15 / vol 120 / pages 2774-2784


Metastatic lymph node 51 [MLN51 ( also known as CASC3)] is a component of the exon junction complex (EJC), which is assembled on spliced mRNAs and plays important roles in post-splicing events. The four proteins of the EJC core, MLN51, MAGOH, Y14 and EIF4AIII shuttle between the cytoplasm and the nucleus. However, unlike the last three, MLN51 is mainly detected in the cytoplasm, suggesting that it plays an additional function in this compartment. In the present study, we show that MLN51 is recruited into cytoplasmic aggregates known as stress granules (SGs) together with the SG-resident proteins, fragile X mental retardation protein ( FMRP), poly( A) binding protein ( PABP) and poly( A)(+) RNA. MLN51 specifically associates with SGs via its C-terminal region, which is dispensable for its incorporation in the EJC. MLN51 does not promote SG formation but its silencing, or the overexpression of a mutant lacking its C-terminal region, alters SG assembly. Finally, in human breast carcinomas, MLN51 is sometimes present in cytoplasmic foci also positive for FMRP and PABP, suggesting that SGs formation occurs in malignant tumours.



mammalian-cells; breast-cancer; breast cancer; barentsz; binding proteins; core complex; cytoplasmic foci; dcp1; fmrp; mental-retardation protein; messenger-rna decay; mln51; p-bodies; recruitment; stress granule; translational repressor

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