Uptake of oligonucleotides by keratinocytes.

Laktionov, P.; Dazard, J. E.; Piette, J.; Vives, E.; Rykova, E.; Vlassov, V.; Lebleu, B.

Nucleosides & Nucleotides

1999 / vol 18 / pages 1697-1699


Oligonucleotides (ODNs) conjugated to rhodamin (Rh) and 4-[(N-2-chloroethyl-N-methyl)amino] benzylamine were used to investigate ODNs transport into keratinocytes. Affinity labeling of two:proteins, 63 and 35 kDa, and the inhibition of the affinity labeling and ODNs uptake by the cells in the presence of nucleic acids, polyanions and trypsin suggest,that the proteins are involved in transport of nucleic acids in keratinocytes.


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