Imprinted genes play essential roles in development, and their allelic expression is mediated by imprinting control regions (ICRs). The Dlk1-Dio3 locus is among the few imprinted domains controlled by a paternally methylated ICR. The unmethylated maternal copy activates imprinted expression early in development through an unknown mechanism. We find that in mouse embryonic stem cells (ESCs) and in blastocysts, this function is linked to maternal, bidirectional expression of noncoding RNAs (ncRNAs) from the ICR. Disruption of ICR ncRNA expression in ESCs affected gene expression in cis, led to acquisition of aberrant histone and DNA methylation, delayed replication timing along the domain on the maternal chromosome, and changed its subnuclear localization. The epigenetic alterations persisted during differentiation and affected the neurogenic potential of the stem cells. Our data indicate that monoallelic expression at an ICR of enhancer RNA-like ncRNAs controls imprinted gene expression, epigenetic maintenance processes, and DNA replication in embryonic cells.
ICR noncoding RNA expression controls imprinting and DNA replication at the Dlk1-Dio3 domain
Kota§, S. K.; Lleres§, D.; Bouschet, T.; Hirasawa, R.; Marchand, A.; Begon-Pescia, C.; Sanli, I.; Arnaud, P.; Journot, L.; Girardot, M.; Feil, R.
2014-10-13 / vol 31 / pages 19-33
10.1016/j.devcel.2014.08.009 S1534-5807(14)00519-X [pii]
1878-1551 (Electronic) 1534-5807 (Linking)
IGMM team(s) involved in this publication
Empreinte Génomique et Développement
Animals; Mice; DNA Methylation; *DNA Replication; *Genomic Imprinting; Epigenesis, Genetic; Cell Differentiation; *Locus Control Region; Blastocyst/cytology/metabolism; Embryonic Stem Cells/cytology/metabolism; Intercellular Signaling Peptides and Proteins/*genetics; Iodide Peroxidase/*genetics; RNA, Untranslated/*genetics/metabolism