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SEMINARS

UPCOMING EVENTS

All seminars take place usually each Tuesday at 11 am at 11:00 AM in the meeting room “Philippe Jeanteur “.

1 December 2022
  • Adrien HALLOU (Gurdon Institute, Cambridge, UK)

    1 December 2022  11 h 00 min - 12 h 00 min
    Centre de Recherche en Biologie Cellulaire de Montpellier (CRBM), 1919 Rte de Mende, 34090 Montpellier, France (Salle Marcel Dorée)

    Spatial mechano-transcriptomics of the early mouse embryo

    Cell morphology, mechanical forces, and gene expression act together to orchestrate cell fate decisions and tissue morphogenesis during embryogenesis. Analysis into the nature of this cooperation is therefore required to fully understand the nature of the highly complex mechanisms which sculpt the developing embryo in space and time. Here, I will present a new and unique computational approach combining image-based mechanical force inference and spatial transcriptomics and demonstrate its applicability to derive at both single cell and tissue level mechanical and morphometric information alongside gene expression levels. Using a seqFISH dataset of the E8.5 mouse embryo, we show that an integrated analysis of these modalities enables resolution of functional cell subtypes that cannot be distinguished by gene expression alone, as well as identification of gene modules predictive of the mechanical and morphological state of a cell, and of the formation of boundaries between tissue compartments. This method can be applied to any spatial transcriptomics dataset with sufficient cell membrane segmentation quality, enabling further analysis into the complex interactions between cell morphology, mechanical forces, and gene expression.

     

    Selected publications

    Deep learning for bioimage analysis in developmental biology A Hallou, HG Yevick, B Dumitrascu and V Uhlmann, Development 148(18) dev199616 (2021) 

     A biomechanical switch regulates the transition towards homeostasis in oesophageal epithelium J McGinn, A Hallou, S Han et al., Nature Cell Biology 23 511 (2021) 

     On growth and force: mechanical forces in development A Hallou and T Brunet, Development 147(4) dev187302 (2020) 

     Theory of mechanochemical patterning in biphasic biological tissues P Recho, A Hallou and E Hannezo, PNAS 116(12) 5344 (2019) 

     Tumour heterogeneity promotes collective invasion and cancer metastatic dissemination A Hallou, J Jennings and AJ Kabla, R. Soc. Open Sci. 4 161007 (2017) 

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6 December 2022
  • ANNULÉ - Claire ROUGEULLE (Université Paris Cité, UMR 7216)

    6 December 2022  11 h 30 min - 12 h 30 min
    Institut de Génétique Moléculaire de Montpellier (IGMM), 1919 Rte de Mende, 34090 Montpellier, France

    Title : "Diving into X chromosome inactivation, from an evolutionary perspective"
    Contact : maud.borensztein@igmm.cnrs.fr

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8 December 2022
  • Simon GEMBLE (Curie Institute, Paris)

    8 December 2022  11 h 00 min - 12 h 00 min
    CNRS-CRBM, 1919 Rte de Mende, 34293 Montpellier, France

     «Size matters»: How genetic stability and nuclear architecture respond to changes in DNA content.

     Wholegenomeduplication(WGD)resultsfromthegainofcompletechromosomesets.WGDcanbedevelopmentallyprogrammedtosustaincellandtissuefunctions.Conversely,whennon-programmed,WGDcangenerategeneticinstability.Indeed,ithasbeenassessedthat40%ofhumantumorsaretetraploid,duetoatleastoneeventofWGD.Tetraploidcontentappearstofuelabnormalkaryotypesfavoringtumorigenesis.However,littleisknownabouthowtetraploidizationgeneratesgeneticinstability.Toinvestigatethisquestion,wehaveinducedWGDindiploidhumancelllinesusingmultiplemeansandfoundthatboth(1)geneticstabilityand(2)nucleararchitectureareimpairedwithinthefirstcellcycleuponWGD.Indeed,(1)wedemonstratedthathighlevelsofDNAdamagearegeneratedinaDNA-replicationmannerwithinthefirstS-phasefollowingWGD.ThisisresultingfromalackofscalingupofmultiplereplicationfactorsthatcreatesanimbalancebetweentheamountofreplicationmachineriesandDNAcontent,generatingreplicationstress(Gembleetal.,Nature,2022).Moreover,(2)weobservedtheappearanceofnucleardeformationsinthefirstG1uponWGD.TheseabnormalnuclearshapesareduetothemicrotubulecytoskeletonthataregeneratingnuclearinvaginationsduringG1.Wearenowinvestigatingthesubsequentconsequencesofnucleardeformationsontetraploidcell(Unpublisheddata).Ourresultsprovideanewframeworktounravelthemultiplewaysbywhichnon-programmedWGDcanimpactgeneticstabilityandcontributetocancer.

     Genetic instability from a single S phase after whole-genome duplication. GembleS et al. Nature. 2022 doi: 10.1038/s41586-022-04578-4. 

    CHRONOCRISIS: When Cell Cycle Asynchrony Generates DNA Damage in PolyploidCells. GembleS, BastoR. Bioessays. 2020 doi: 10.1002/bies.202000105. 

    https://www.crbm.cnrs.fr/wp-content/uploads/2022/12/Simon-Gemble-affiche.pdf

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13 December 2022
  • Sarah LAMBERT (Institut Curie, Orsay)

    13 December 2022  11 h 00 min - 12 h 30 min
    Institut de Génétique Moléculaire de Montpellier (IGMM), 1919 Rte de Mende, 34090 Montpellier, France

    Title : "Maintaining replication fork integrity and competence in space and time"
    Contact : etienne.schwob@igmm.cnrs.fr

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