The chromosome conformation capture (3C) technique is fundamental to many population-based methods investigating chromatin dynamics and organization in eukaryotes. Here, we provide a modified quantitative 3C (3C-qPCR) protocol for improved quantitative analyses of intra-chromosomal contacts. We also describe an algorithm for data normalization which allows more accurate comparisons between contact profiles.
Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method
Ea, V.; Court, F.; Forne, T.
Methods Mol Biol
2017 / vol 1589 / pages 75-88
1940-6029 (Electronic) 1064-3745 (Linking)
IGMM team(s) involved in this publication
Architecture Génomique et Contrôle Epigénétique
Chromatin dynamics and organization; Chromosome conformation capture; Quantitative PCR