Butterworth, Grégoire and colleagues used single molecule fluorescent in situ hybridization to detect hepatitis C virus (HCV) RNA in liver sections of a patient with hepatocellular carcinoma on hepatitis C background. Laser microdissection followed by RNAseq analysis of clusters of infected hepatocytes revealed HCV-related deregulation of genes expression. This work, carried out in the group led by Urszula Hibner, in collaboration with Edouard Bertrand and Jean-Christophe Andrau ‘ labs at the IGMM and with Joachim Lupberger’s team in Strasbourg, led to the identification of GOLT1B as a cellular factor important for viral replication.
Butterworth J.*/ Grégoire D.*&, Peter M., Roca Suarez AA., Desandré G., Simonin Y., Virzi A., Zine El Aabidine A., Guivarch M., Andrau JC., Bertrand E., Assenat E., Lupberger J., Hibner U.& GOLT1B Activation in Hepatitis C Virus-Infected Hepatocytes Links ER Trafficking and Viral Replication, Pathogens, 2022, 11,46. https:// doi.org/10.3390/pathogens11010046.