To initiate studies on how protein-protein interaction (or “interactome”) networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains similar to5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.
A map of the interactome network of the metazoan C-elegans
Li, S. M.; Armstrong, C. M.; Bertin, N.; Ge, H.; Milstein, S.; Boxem, M.; Vidalain, P. O.; Han, J. D. J.; Chesneau, A.; Hao, T.; Goldberg, D. S.; Li, N.; Martinez, M.; Rual, J. F.; Lamesch, P.; Xu, L.; Tewari, M.; Wong, S. L.; Zhang, L. V.; Berriz, G. F.; Jacotot, L.; Vaglio, P.; Reboul, J.; Hirozane-Kishikawa, T.; Li, Q. R.; Gabel, H. W.; Elewa, A.; Baumgartner, B.; Rose, D. J.; Yu, H. Y.; Bosak, S.; Sequerra, R.; Fraser, A.; Mango, S. E.; Saxton, W. M.; Strome, S.; van den Heuvel, S.; Piano, F.; Vandenhaute, J.; Sardet, C.; Gerstein, M.; Doucette-Stamm, L.; Gunsalus, K. C.; Harper, J. W.; Cusick, M. E.; Roth, F. P.; Hill, D. E.; Vidal, M.
2004
Science
2004-01-23 / vol 303 / pages 540-543
Abstract
0036-8075
Tags
gene-expression; saccharomyces-cerevisiae; identification; genome; drosophila; caenorhabditis-elegans; protein-protein interactions; complexes; eye development; information