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Crosstalk between mRNA 3′ end processing and transcription initiation

Mapendano, C. K.; Lykke-Andersen, S.; Kjems, J.; Bertrand, E.; Jensen, T. H.

Mol Cell

2010-11-12 / vol 40 / pages 410-22

Abstract

Transcription and mRNA maturation are interdependent events. Although stimulatory connections between these processes within the same round of transcription are well described, functional coupling between separate transcription cycles remains elusive. Comparing time-resolved transcription profiles of single-copy integrated beta-globin gene variants, we demonstrate that a polyadenylation site mutation decreases transcription initiation of the same gene. Upon depletion of the 3′ end processing and transcription termination factor PCF11, endogenous genes exhibit a similar phenotype. Readthrough RNA polymerase II (RNAPII) engaged on polyadenylation site-mutated transcription units sequester the transcription initiation/elongation factors TBP, TFIIB and CDK9, leading to their depletion at the promoter. Additionally, high levels of TBP and TFIIB appear inside the gene body, and Ser2-phosphorylated RNAPII accumulates at the promoter. Our data demonstrate that 3′ end formation stimulates transcription initiation and suggest that coordinated recycling of factors from a gene terminator back to the promoter is essential for sustaining continued transcription.

Read on PubMed

10.1016/j.molcel.2010.10.012 S1097-2765(10)00788-4 [pii]

1097-4164 (Electronic) 1097-2765 (Linking)

IGMM team(s) involved in this publication
Tags

Humans; HEK293 Cells; *Transcription, Genetic; Models, Biological; RNA Polymerase II/metabolism; Promoter Regions, Genetic/genetics; Molecular Sequence Data; Phenotype; Phosphorylation; RNA Splicing/genetics; Base Sequence; Time Factors; RNA, Messenger/genetics/*metabolism; Cyclin-Dependent Kinase 9/metabolism; mRNA Cleavage and Polyadenylation Factors/metabolism; Phosphoserine/metabolism; Point Mutation/genetics; Poly A/genetics; RNA 3' End Processing/*genetics; TATA-Box Binding Protein/metabolism

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