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Interaction between the small-nuclear-RNA cap hypermethylase and the spinal muscular atrophy protein, survival of motor neuron

Mouaikel, J.; Narayanan, U.; Verheggen, C.; Matera, A. G.; Bertrand, E.; Tazi, J.; Bordonne, R.

EMBO Rep

2003-06 / vol 4 / pages 616-22

Abstract

The biogenesis of spliceosomal small nuclear ribonucleoproteins (snRNPs) requires the cytoplasmic assembly of the Sm-core complex, followed by the hypermethylation of the small nuclear RNA (snRNA) 5′ cap. Both the Sm-core complex and the snRNA trimethylguanosine cap are required for the efficient nuclear import of snRNPs. Here, we show that trimethylguanosine synthase 1 (TGS1), the human homologue of the yeast snRNA cap hypermethylase, interacts directly with the survival of motor neuron (SMN) protein. Both proteins are similarly distributed, localizing in the cytoplasm and in nuclear Cajal bodies. The interaction between TGS1 and SMN is disrupted by a mutation in SMN that mimics the predominant isoform of the protein that is expressed in patients with the neurodegenerative disease, spinal muscular atrophy. These data indicate that, in addition to its function in cytoplasmic Sm-core assembly, the SMN protein also functions in the recruitment of the snRNA cap hypermethylase.

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Tags

Humans; Models, Biological; Protein Binding; Ribonucleoproteins, Small Nuclear/*chemistry/metabolism; Immunohistochemistry; Precipitin Tests; Tissue Distribution; Cell Line; Autoantigens; Blotting, Western; Protein Isoforms; Electrophoresis, Polyacrylamide Gel; Hela Cells; Cytoplasm/metabolism; Plasmids/metabolism; Cloning, Molecular; Glutathione Transferase/metabolism; RNA, Small Nuclear/*metabolism; Methyltransferases/*chemistry/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; RNA Cap-Binding Proteins

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