The polymerase chain reaction using oligonucleotides based on consensus sequences from other species allowed us to amplify minke whale (Balaenoptera acutorostrata) T cell receptor (TcR) gamma and delta constant genes. Two types of C-gamma clones were obtained which only differ by one mismatch. These minke whale C-gamma clones showed 83% nucleotide and 70% amino acid sequence similarity to the corresponding region of the human TRGC genes. The minke whale C-delta sequences were all identical, and showed 86% nucleotide and 77.8% amino acid sequence similarity to the human TRDC gene. The whale C-gamma and C delta clones were used as probes for Southern blot analysis. The data confirmed that there is a unique C-delta gene in minke whale. Two different C-gamma genes were detected, one of them also cross-hybridizing with a human C-gamma probe, suggesting that the C-gamma locus in minke whale consists of at least two different constant genes.
Isolation of T cell receptor gamma/delta chain constant genes from minke whale
Zhang, X. M.; Huck, S.; Dariavach, P.; Spilliaert, R.; Arnason, A.; Soua, Z.; Lefranc, G.; Lefranc, M. P.
Experimental and Clinical Immunogenetics
1995 / vol 12 / pages 245-252
organization; expression; locus; rearrangement; t cell receptor; alpha; diversity; gamma-delta; molecular-cloning; region genes; sequence; whale