IL-7 differentially regulates cell cycle progression and HIV-1-based vector infection in neonatal and adult CD4+ T cells

Dardalhon, V.; Jaleco, S.; Kinet, S.; Herpers, B.; Steinberg, M.; Ferrand, C.; Froger, D.; Leveau, C.; Tiberghien, P.; Charneau, P.; Noraz, N.; Taylor, N.

Proc Natl Acad Sci U S A

2001-07-31 / vol 98 / pages 9277-82


Differences in the immunological reactivity of umbilical cord (UC) and adult peripheral blood (APB) T cells are poorly understood. Here, we show that IL-7, a cytokine involved in lymphoid homeostasis, has distinct regulatory effects on APB and UC lymphocytes. Neither naive nor memory APB CD4(+) cells proliferated in response to IL-7, whereas naive UC CD4(+) lymphocytes underwent multiple divisions. Nevertheless, both naive and memory IL-7-treated APB T cells progressed into the G(1b) phase of the cell cycle, albeit at higher levels in the latter subset. The IL-7-treated memory CD4(+) lymphocyte population was significantly more susceptible to infection with an HIV-1-derived vector than dividing CD4(+) UC lymphocytes. However, activation through the T cell receptor rendered UC lymphocytes fully susceptible to HIV-1-based vector infection. These data unveil differences between UC and APB CD4(+) T cells with regard to IL-7-mediated cell cycle progression and HIV-1-based vector infectivity. This evidence indicates that IL-7 differentially regulates lymphoid homeostasis in adults and neonates.

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Humans; Flow Cytometry; Cell Cycle/*physiology; CD4-Positive T-Lymphocytes/*immunology; Cell Division; HIV-1/*genetics; *Genetic Vectors; Interleukin-7/*physiology; T-Lymphocyte Subsets

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