close

Lipophilic pro-oligonucleotides are rapidly and efficiently internalized in HeLa cells

Vives, E.; Dell'Aquila, C.; Bologna, J. C.; Morvan, F.; Rayner, B.; Imbach, J. L.

Nucleic Acids Research

1999-10-15 / vol 27 / pages 4071-4076

Abstract

Model t-Bu-SATE pro-dodecathymidines labeled with fluorescein and exhibiting various lipophilicities were evaluated for their uptake by cells in culture. Pro-oligonucleotides with appropriate lipophilicity were found to permeate across the HeLa cell membrane much more extensively than the control phosphorothioate oligo or than the hydrophilic prooligos. Fluorescence patterns of internalization were consistent with a diffusion mechanism resulting in the appearance of a uniform cytoplasmic distribution and nuclear accumulation, as confirmed by confocal microscopy.

0305-1048

Tags

solid-phase synthesis

Back to all publications