Polyimidazole conjugated oligonucleotides reach the nucleus of HeLa cells

Morvan, F.; Castex, C.; Vives, E.; Imbach, J. L.

Nucleosides Nucleotides & Nucleic Acids

2001 / vol 20 / pages 805-808


Oligonucleotide models bearing 6, 12 or 18 histamine residues were synthesized on solid support and labeled with fluorescein. Only the oligo with 6 histamine residues showed a high uptake in HeLa cells with a nuclear localization. Experiment a 4 degreesC or with bafilomicyn A(1) suggest that uptake proceeded by an endocytosis mechanism followed by a destabilization of the membrane. Once in the cytoplasm the oligo reached rapidly the nucleus.




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