The H19/IGFf2 locus belongs to a large imprinted domain located on human chromosome 11p15.5 (homologue to mouse distal chromosome 7). The H19 gene is expressed from the maternal allele, while IGF2 is paternally expressed. Natural antisense transcripts and intergenic transcription have been involved in many aspects of eukaryotic gene expression, including genomic imprinting and RNA interference. However, apart from the identification of some IGF2 antisense transcripts, few data are available on that topic at the H19/IGF2 locus. We identify here a novel transcriptional activity at both the human and the mouse H19/IGF2 imprinted loci. This activity occurs antisense to the H19 gene and has the potential to produce a single 120-kb transcript that we called the 91H RNA. This nuclear and short-lived RNA is not imprinted in mouse but is expressed predominantly from the maternal allele in both mice and humans within the H19 gene region. Moreover, the transcript is stabilized in breast cancer cells and overexpressed in human breast tumors. Finally, knockdown experiments showed that, in humans, 91H, rather than affecting H19 expression, regulates IGF2 expression in trans.
A novel H19 antisense RNA overexpressed in breast cancer contributes to paternal IGF2 expression
Berteaux, N.; Aptel, N.; Cathala, G.; Genton, C.; Coll, J.; Daccache, A.; Spruyt, N.; Hondermarck, H.; Dugimont, T.; Curgy, J. J.; Forne, T.; Adriaenssens, E.
Mol Cell Biol
2008-11 / vol 28 / pages 6731-45
MCB.02103-07 [pii] 10.1128/MCB.02103-07
IGMM team(s) involved in this publication
Genome Organization and Epigenetic Control
Female; Humans; Animals; Mice; Male; Molecular Sequence Data; RNA Interference; Models, Genetic; Genomic Imprinting; Base Sequence; *Gene Expression Regulation, Neoplastic; Transcription, Genetic; RNA, Untranslated/genetics/*metabolism; Breast Neoplasms/*genetics/metabolism; DNA, Intergenic/genetics; Insulin-Like Growth Factor II/genetics/*metabolism; RNA, Antisense/genetics/*metabolism