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Regulation of gene expression during development

Mounia Lagha

Research projects

During development, precise control of gene expression allows the reproducible establishment of patterns, leading to the formation of organs at the right time and place. What are the mechanisms behind such precision?

The establishment of developmental patterns has been primarily studied at the transcriptional level. In comparison, the fate of these transcripts received little attention. Development of new methods enabling the study of translation of single mRNA molecules revealed a critical heterogeneity in translation. Our lab pioneered the deployment of such methods in Drosophila embryos, leading to the discovery of translation factories and unmasking intragenic translation heterogeneity, demonstrating a novel layer of variation in gene expression. Thus, to decipher the mechanisms dictating precision in gene expression, it is essential to simultaneously consider both layers of regulation constituted by transcription and translation as well as their potential coupling.

We propose to bridge the gap between these two pillars of the central dogma to obtain an integrated multiscale view of gene expression control. We use quantitative imaging methods to simultaneously monitor mRNA and protein in living Drosophila embryos with unprecedented spatio-temporal resolution, combined with theoretical models to formulate predictions that will be tested by genetic/optogenetic manipulations.

 

PROJECT 1

How do translation dynamics of localized patterning transcripts modulate cell fate decisions?

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PROJECT 2
How are transcription and translation processes coupled?

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PROJECT 3

Nuclear mobilities of cis-regulatory hubs in transcriptional control

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PROJECT 4
Impact of cis-reg sequences on transcriptional noise during development

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Members

Team leader

Mounia LAGHA

Chercheur DR2

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104

Matthieu ANGLES

IE-Recherche

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104

Ronan BOUZIGNAC

Doctorant

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104

Maria DOUAIHY

Doctorant

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Louise MAILLARD

Doctorant

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104

Virginia PIMMETT

Post-Doc

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104

Amandine PALANDRI

IE-Recherche

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104

Antonio TRULLO

IR-Recherche

+33 (0)4 34 35 96 50

Morgane VERBRUGGHE

IE-Recherche

+33 (0)4 34 35 96 53

104

Previous lab members

Matthieu Dejean / Research Assistant

Marion Goussard /  Research Assistant

Maelys Gicquel / Research Assistant

Carola Fernandez / PhD Student

Jennifer Hunter / Post-Doc

Yayoi Wada / trainee

Elisa Atger / trainee

Rachida el Youssfi / trainee

Matilde Gauchier / trainee

Matilde Montovani / trainee

Louis Redonnet / trainee

Jonathan Lapine / trainee

Quentin Dessables / trainee

Yasmine Hadj-Messaoud / trainee

Matilde Mantovani / trainee

Yayoi Wada /  trainee

Quantin Dessables /  trainee

Mathilde Gauchier /  trainee

Louis Redonnet / trainee

Rachida el Youssfi / trainee

Elisa Atger / trainee

Hoa Vinh Le /  trainee

Saida Nait Amer / trainee

Lucas Makinen / trainee

Basile Poutier / trainee

Lucas Morales / trainee

Jorge Lazaro / trainee

Olivier Messina / trainee

Heloise Faure-Gautron / trainee

Selected Publications

More information

Funding

2020              

ANR MemoRNP (with F.Besse)

2020             

CNRS-Chicago Prime80 (with J.Reinitz and O.Radulescu)

2020             

LabMuse PhD network (3 PhD fellowships with E.Bertrand, S.Chambeyron and O.Radulescu)

2018

FRM fellowship to support M.Bellec PhD under my supervision

2015              

FRM grant to support the salary of a research engineer

2015              

ERC starting grant, SynDev

 

Interactions
In Montpellier

O.Radulescu and L.Ciandrini (Univ.Montpellier) : mathematical modeling

National and international

M.Harrison (University of Wisconsin Madison, USA): Role of Zelda

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Team Overview
Model organism studied
Drosophila Melanogaster
Biological process
Gene expression, transcriptional dynamics
Biological techniques
Quantitative live imaging, genetics